When Puss in Boots (Antonio Banderas) discovers he's used up eight of his nine lives, his doctor recommends he retire and refrain from adventuring. Unhappy with that request, Puss sets out on a journey with Kitty (Salma Hayek) and a stray dog they name Perrito, after retrieving a map bearing the location of the Wishing Star.

The map sends the trio to the Dark Forest to find the mythical Last Wish and restore all of Puss's nine lives.